Yvon Woappi, Ph.D.
Assistant Professor, Department of Physiology and Cellular Biophysics
Herbert and Florence Irving Assistant Professor of Dermatology
Affiliate faculty: Department of Biomedical Engineering.
Multilineage restoration of tissue wounds; Systems physiology
of wound healing.
The Woappi lab for Synthetic Regeneration and Systems Physiology aims to understand how varied cells mediate restoration of damaged tissue, with the goal of leveraging this insight to develop novel treatments for large-scale tissue injuries. We study tissue as a system and develop gene-editing tools and synthetic biotechnologies, along with 3D skin culture systems, to delineate the genetic, epigenetic, and biophysical events orchestrating distinct cells towards restoration of tissue. Our laboratory has three major research interests: 1) Defining the biophysical mediators of wound healing by developing microphysiological systems (MPS) of wound mimicry, 2) Developing synthetic biotechnologies to examine the regeneration potential of mammalian tissue,
3) Characterizing the immunological responses to wound injury.
Our research established the human skinoid culture system, which enables spatiotemporal modeling of human tissue reconstruction ex vivo. We are furthering this work by creating microphysiological systems mimicking human wound healing and using these platforms to model the cellular heterogeneity of tissue healing .
Our team is motivated to define the molecular, genetic, and epigenetic events enabling heterogenous cells to orchestrate restoration of damaged tissue. We are developing novel synthetic biotechnologies to functionally dissect the role of specific cell populations in tissue maintenance, neoplasia, and wound healing.
Systems Immunology of Wounds
Our research seeks to understand how localized immune cells enable proximal and distal orchestration of tissue stem cells to a healing state. Our group employs quantitative systems biology to uncover novel immune contributions to tissue healing. We leverage this insight to develop synthetic immunosurveillance systems (S.I.S) with prophylactic and therapeutic potentials for wound patients.
Boudra, R; Woappi, Y*; Wang, D; Xu, S; Wells, M; Schmults, C; Lian, C; Ramsey, R. Regulation of 5-hydroxymethylcytosine by TET2 contributes to squamous cell carcinoma tumorigenesis. Journal of Investigative Dermatology (2021). DOI: 10.1016/j.jid.2021.09.026 *= co-first author
Woappi, Y*; Altomare, D; Creek, K; and Pirisi, L. Self-assembling 3D spheroid cultures of human neonatal keratinocytes have enhanced regenerative properties. Stem Cell Research (2020). DOI: 10.1016/j.scr.2020.102048 *= co-first author
Feng, Li; Yuan, C; Xu, S; Zu, T; Woappi, Y, Lee, C; Abarzua, P; Wells, M; Ramsey, M; Frank, N; Wu, X; Mandinova, N; Frank, M; Lian, C; and Murphy, G. Loss of the Epigenetic Mark 5-hmC in Psoriasis: Implications for Epidermal Stem Cell Dysregulation (2020). Journal of Investigative Dermatology. DOI: 10.1016/j.jid.2019.10.016
Woappi, Y*; Hosseinipour, M; Creek, K; and Pirisi, L. Stem Cell Properties of Normal Human Keratinocytes Determine Transformation Responses to Human Papillomavirus 16 DNA (2018), Journal of Virology. DOI: https://doi.org/10.1128/JVI.00331-18 *= co-first author
Woappi, Y*; Jangiti, R., Singh, Om. Synthetic immunosurveillance systems: Nanodevices to monitor physiological events (2014). DOI: 10.1016/j.bios.2014.04.056